Human C1q R1 / CD93 Protein, His Tag (MALS verified) ยี่ห้อ Acrobiosystems

รหัสสินค้า : C11-H5228-50ug

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Synonym

  • CD93, CDw93, C1QR1, MXRA4, C1qR, C1qR(P), C1qRP, ECSM3

Source

  • Human C1q R1, His Tag(C11-H5228) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Lys 580 (Accession # AAH28075 ).


Molecular Characterization

  • This protein carries a polyhistidine tag at the C-terminus
  • The protein has a calculated MW of 59.2 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.


Endotoxin

  • Less than 1.0 EU per μg by the LAL method.


Purity

  • >90% as determined by SDS-PAGE.
  • >90% as determined by SEC-MALS.


Formulation

  • Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
  • Contact us for customized product form or formulation.


Reconstitution

  • Please see Certificate of Analysis for specific instructions.
  • For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.


Storage

  • For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Please avoid repeated freeze-thaw cycles.
  • This product is stable after storage at:
  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.


SDS-PAGE

C1q R1 SDS-PAGE

Human C1q R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.


Bioactivity-ELISA

 C1q R1 ELISA

Immobilized Human C1q R1, His Tag (Cat. No. C11-H5228) at 5 μg/mL (100 μL/well) can bind Human IGFBP-7, Fc Tag (Cat. No. IG7-H5259) with a linear range of 0.6-20 ng/mL (QC tested).


Bioactivity-SPR

 C1q R1 SPR

Human IGFBP-7, His Tag (Cat. No. IG7-H5240) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. C11-H5228) with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).


SEC-MALS

C1q R1 MALS images

The purity of Human C1q R1, His Tag (Cat. No. C11-H5228) is more than 90% and the molecular weight of this protein is around 75-85 kDa verified by SEC-MALS.

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