Human C1q R1 / CD93 Protein, His Tag (MALS verified) ยี่ห้อ Acrobiosystems
รหัสสินค้า : C11-H5228-50ug
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Synonym
- CD93, CDw93, C1QR1, MXRA4, C1qR, C1qR(P), C1qRP, ECSM3
Source
- Human C1q R1, His Tag(C11-H5228) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Lys 580 (Accession # AAH28075 ).
Molecular Characterization
- This protein carries a polyhistidine tag at the C-terminus
- The protein has a calculated MW of 59.2 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
- Less than 1.0 EU per μg by the LAL method.
Purity
- >90% as determined by SDS-PAGE.
- >90% as determined by SEC-MALS.
Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
- Contact us for customized product form or formulation.
Reconstitution
- Please see Certificate of Analysis for specific instructions.
- For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
- For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Please avoid repeated freeze-thaw cycles.
- This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human C1q R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human C1q R1, His Tag (Cat. No. C11-H5228) at 5 μg/mL (100 μL/well) can bind Human IGFBP-7, Fc Tag (Cat. No. IG7-H5259) with a linear range of 0.6-20 ng/mL (QC tested).
Bioactivity-SPR
Human IGFBP-7, His Tag (Cat. No. IG7-H5240) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. C11-H5228) with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
SEC-MALS
The purity of Human C1q R1, His Tag (Cat. No. C11-H5228) is more than 90% and the molecular weight of this protein is around 75-85 kDa verified by SEC-MALS.
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