Trizma® base, anhydrous, free-flowing, Redi-Dri™, ≥99.9% ยี่ห้อ Sigma

PROPERTIES

grade

anhydrous

Quality Level

100

description

aminopeptidase substrate

product line

Redi-Dri^™

Assay

≥99.9%

form

powder

quality

free-flowing

impurities

≤0.2% Water (Karl Fischer)

color

white

pH

10.5-12

7-9

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (Water < 0.2%)

absorption

≤0.05 at 290 nm at 40%

λ

(A290 UV absorbance < 0.05)

in 5 mM Tris; DNA (40% (w/w))

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

DESCRIPTION

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[3]

Application

Tris base has been used:

·       To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization[4]

·       In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting[5]

·       In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein[6]

·       As a component of nuclei extraction buffer[7]

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.

For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

SAFETY INFORMATION

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable