PCR
PCR
From endpoint PCR to quantitative real-time PCR and RT-PCR, we offer a complete portfolio of high-quality products to meet your amplification needs. Endpoint and real-time PCR products feature GoTaq® (Taq) DNA polymerase in convenient master mixes, kits and flexible enzyme formulations for basic PCR, hot-start PCR, long-range PCR, qPCR and RT-qPCR.
RT-PCR and real-time RT-qPCR product lines include GoTaq® DNA polymerase and GoScript™ reverse transcriptase, providing robust and reliable reverse transcription and PCR performance for difficult templates and in the presence of PCR inhibitors.
All PCR systems, enzymes and reagents come with our PCR performance guarantee. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
RT-PCR <-- คลิกเพื่อดูรายละเอียดสินค้า
Promega reverse transcription-PCR (RT-PCR) products include standalone reverse transcriptases (AMV, M-MLV and GoScript) and a range of complete kits containing all the required enzymes, buffers and reagents for successful RT-PCR.
PCR Cloning <-- คลิกเพื่อดูรายละเอียดสินค้า
T-vector cloning, or TA cloning, is a convenient method for cloning PCR products generated with Taq DNA Polymerase. The pGEM®-T vectors are a popular choice for general PCR cloning. These vectors are ready to use in ligation reactions; prepared by cutting with a restriction endonuclease that creates a blunt end and adding a 3´ terminal thymidine (T) to both ends. The 3´ T-overhangs at the insertion site greatly improve ligation efficiency of a PCR product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for PCR products with 5' A-overhangs. The pGEM®-T Easy Vector systems come with competent cells included.
Taq Polymerase <-- คลิกเพื่อดูรายละเอียดสินค้า
High-performance Taq polymerase, dNTPs, Buffers and Master Mixes provide increased reliability and consistency for routine endpoint PCR. GoTaq® products offer a choice of Taq DNA polymerase formulations covering basic PCR, hot-start PCR and long-range PCR applications.
qPCR and RT-qPCR <-- คลิกเพื่อดูรายละเอียดสินค้า
Promega real-time PCR (qPCR) and RT-qPCR assays provide a choice of probe-based or dye-based qPCR methods. We also offer XpressAmp™ Reagents that are compatible for use with the GoTaq® qPCR and RT-qPCR Systems and enable direct PCR amplification from viral samples without having to perform RNA or DNA extraction.
