SMC® Human IFN-γ High Sensitivity Immunoassay Kit ยี่ห้อ Sigma

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General description

Interferon gamma is a pro-inflammatory cytokine produced by activated T lymphocytes and natural killer cells. It is important in the activation of macrophages and MHC expression and plays a key role in both inflammation and autoimmune disease by enhancing antigen presentation, increasing leukocyte trafficking and affecting cell proliferation and apoptosis.

The SMC^® Human IFN-γ High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.

Assay protocols are similar to existing sandwich ELISA methods with two key differences:

1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.

2) The Erenna and SMCxPRO^® immunoassay systems detect analytes using Single Molecule Counting (SMC^®) technology.

Specificity

Species Reactivity:

Although IFN-γ was detectable in rat, mouse, non-human primate, feline, and canine plasma, the assay has not been validated for these species.

Cross Reactivity:

The SMC^® Human IFN-γ High Sensitivity Immunoasay Kit was not found to be cross-reactive with the following:

·      rhIFN-α2

·      rhIFN-β

·      rhIFN-λ1 (IL-29)

·      rhIFN-λ2 (IL-28A)

·      rhIFN-λ3 (IL-28B)

·      rhIFN-ω2

Accuracy:

Minimum of 5 serum samples spiked with average recovery of 104% with a range of 92-124%

Minimum of 5 plasma samples spiked with average recovery of 98% with a range of 87-110%

Application

The sensitivity of the Single Molecule Counting (SMC^® technology gives the SMC^® Human IFN-γ High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-γ in human serum and plasma.

This new streamline kit has been improved with the following features over the legacy IFN-γ kit, #03-0049-00:

·      LLOQ has been lowered from 0.2 pg/mL in the legacy kit to 0.03 pg/mL in the new kit

·      Improved options for sample prep and incubation

·      Lyophilization of the standard provides the kit at one storage condition

·      Removal of a plate transfer step

·      Increased reagent volumes to accommodate automation

·      Validated to run on both SMCxPRO^® and Erenna

·      Tested against WHO standard

Note: Recombinant protein is not stable and vortexing is not recommended.

Research Category:

Inflammation & Immunology

Research Sub-Category:

·      Inflammatory Disease

·      Immune Response

·      Autoimmune disease

Packaging

96 well plate

Components

·      Assay Buffer

·      IFN-γ Coated Beads

·      Standard Diluent

·      IFN-γ Detection Antibody

·      IFN-γ Standard

·      10X Wash Buffer

·      Buffer D

·      Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.