Anti-Mouse IgG Antibody, HRP conjugate; produced in goat; Purified goat polyclonal IgG in buffer containing conjugated to horseradish peroxidase lyophilized ยี่ห้อ Sigma

PROPERTIES

biological source

goat

Quality Level

100

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Upstate^®

technique(s)

ELISA: suitable

immunohistochemistry: suitable

western blot: suitable

shipped in

dry ice

DESCRIPTION

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Recognizes mouse IgG, both heavy and light chains.

Immunogen

Highly purified whole mouse IgG.

Application

Additional Research Applications

ELISA and Immunohistochemistry:

A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.

Optimal antibody dilution for other applications should be determined by the researcher.

Goat Anti-Mouse IgG Antibody, HRP conjugate is an antibody against Mouse IgG for use in ELISA, IH & WB.

Research Category

Secondary & Control Antibodies

Research Sub Category

Whole Immunoglobulin Secondary Antibodies

Quality

Western Blot Analysis:

Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.

Physical form

Purified goat polyclonal IgG in buffer containing conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, and 0.01% gentamicin sulfate.

Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

SAFETY INFORMATION

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable