Chloramphenicol, ยี่ห้อ Sigma

DESCRIPTION

General description

Synthetic bacteriostatic antibiotic that inhibits the translation of RNA by blocking the peptidyltransferase reaction on ribosomes.

Synthetic bacteriostatic antibiotic that inhibits the translation of RNA to protein by binding to the 50S ribosomal subunit, blocking the peptidyl transferase reaction. Chloramphenicol resistance is encoded by the resistance gene cat, whose product, chloramphenicol acetyltransferase, inactivates the antibiotic via acetylation. Typically used at 30-50 µg/ml for selection of chloramphenicol-resistant Escherichia coli.

Packaging

25 g in Plastic ampoule

100, 500 g in Glass bottle

Biochem/physiol Actions

Potency: ≥970 µg/mg

Primary Target

trasnslation of RNA

Other Notes

Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1, (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.

Lu, J., and Jiang, C. 1993. Biochem. Biophys. Res. Commun.196, 12.

Saltarelli, M.J., et al. 1993. Virol.197, 35.

Holt, J.T. 1992. Ann. N.Y. Acad. Sci.660, 88.

Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, NY, p. 1.6, A.6.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany