DL-Dithiothreitol (DTT),BioUltra, for molecular biology, 1 M in H2O ยี่ห้อ Sigma

PROPERTIES

grade

for molecular biology

Quality Level

200

product line

BioUltra

form

liquid

concentration

~1 M in H₂O

impurities

DNases, none detected

RNases, none detected

phosphatases, none detected

proteases, none detected

pH

2.7-5.0

density

1.04 g/mL at 20 °C

absorption

cut-off at 330 nm

suitability

in accordance for filter test

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

DESCRIPTION

Application

DL-dithiothreitol solution has been used:

·       in the treatment of intestinal explants[1]

·       in homogenate preparation of gill cells for enzymatic assays[2]

·       in western blotting[3]

·       in transglutaminase activity[4]

Biochem/physiol Actions

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms.[5] It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.[6]