MILLIPLEX® Multi-Pathway Total Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay ยี่ห้อ Sigma

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General description

The MILLIPLEX^® ColA MAP 9-plex Multi-Pathway Total Magnetic Bead kit is used to detect total protein levels for ERK/MAP kinase 1/2, Akt, STAT3, JNK, p70 S6 kinase, NFκB, STAT5A/B, CREB, and p38 in cell lysates using the Luminex^® xMAP technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.Analytes available:Erk/MAPK 1/2 (Total);Akt (Total);STAT3 (Total);JNK (Total);p70 S6 Kinase (Total);NFκB (Total);STAT5A/B (Total);CREB (Total);p38 (Total)

Storage and Stability

Recommended storage for kit components is 2 - 8°C

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.