GUANIDINE HCL OMNIPUR, ยี่ห้อ MERCK

DESCRIPTION

General description

Denaturant used for purification of nucleic acids and polypeptides from cell lysates. Inhibits RNase and protease activity. Molecular Biology grade.br

A260 (0.1 M, water): ≤0.05 AU

A280 (0.1 M, water): ≤0.01 AU

Purity by Assay: ≥99.5%

DNase: None detected

Heavy metals (as Pb): ≤0.001%

Phosphatase: None detected

Protease: None detected

RNase: None detected

Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany