ProteoExtract® Complete Mammalian Protein Extraction Kit ยี่ห้อ MERCK

PROPERTIES

usage

20 extractions

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

avoid repeated freeze/thaw cycles

input

sample type: mammalian tissue(s)

sample type: mammalian cultured cells

shipped in

ambient

storage temp.

2-8°C

DESCRIPTION

General description

A complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss. The extraction results in a total protein sample that is a clarified and non-viscous protein solution showing higher resolution and an increased total number of spots in 2-D gel electrophoresis. Sample size: 1x10⁷ - 1x10⁸ cells or 50-100 mg tissue.

Packaging

1 kit in Glass bottle

Components

Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase^® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS

Principle

ProteoExtract^® Complete Mammalian Proteome Extraction Kit is designed to extract total proteins from mammalian tissue culture cells or mammalian tissue.

Storage and Stability

·       Wash Buffer

The Wash Buffer is provided ready-to-use. The buffer should be stored at 4°C.

·       Resuspension Buffer

The Resuspension Buffer is provided ready-to-use. It can be stored at 4°C up to 6 month. For prolonged storage freeze the buffer in convenient aliquots at -20°C.

Avoid repeated freezing and thawing!

·       Extraction Reagent

The Extraction Reagent is supplied as dry powder and can be stored unopened at 4°C. The Extraction Reagent should be reconstituted just prior to use as described below. After reconstitution, it can be stored at 4°C up to 6 month. For prolonged storage freeze the buffers in convenient aliquots at -20°C.

Avoid repeated freezing and thawing!

·       Reducing Agent

The Reducing Agent is supplied as dry powder. It can be stored unopened at 4°C. The Reducing Agent should be reconstituted just prior to use as described below. After reconstitution, aliquot the unused material in 60 µl volumes and freeze at -20°C for further use.

Avoid repeated freezing and thawing!

·       Benzonase^®

Benzonase^® should be stored at 4°C.

Other Notes

Yuan, X., et al. 2002. Electrophoresis 23, 1185. Allen, L. 2000. Functional Genomics Nature 405 819. Herbert, B. 1999. Electrophoresis 20, 660.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany