cOmplete™ Tablets ULTRA, mini, EDTA-free, EASYpack 30 tablets ยี่ห้อ Roche

PROPERTIES

form

tablet

packaging

pkg of 30 tablets (individually packed in foil blister packs)

manufacturer/tradename

Roche

technique(s)

immunoblotting: suitable

western blot: suitable

solubility

water: soluble

absorption

0.4 at 280 nm (1x solution)

storage temp.

2-8°C

DESCRIPTION

General description

Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. However, metalloproteases are prevalent in (for example) bacteria and some techniques require that EDTA be omitted.

cOmplete ULTRA Tablets, Mini, EDTA-free contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA Tablet, Mini, EDTA-free is sufficient for a volume of 10 ml extraction solution.

cOmplete ULTRA Tablets, Mini, EDTA-free are identical to cOmplete ULTRA Tablets, Mini, the only difference being that no EDTA or other chelating agents (e.g. EGTA) are included.

Specificity

Inhibits serine proteases, cysteine proteases, and aspartic proteases. Does not inhibit metalloproteases.

Application

During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete^™ ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows highest inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.

This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for maximum efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.[1][2][3]

This tablet does not contain EDTA; therefore, metalloproteases are not inhibited.

For the purification of histidine-tagged proteins using Immobilized Metal Ion Affinity Chromatography (IMAC), this EDTA-free tablet is recommended for applications using non-EDTA compatible IMAC resins. Alternatively, an EDTA-containing cOmplete ULTRA, Mini tablet may be used in combination with Roche ′s new EDTA-compatible chromatography material, cOmplete His-Tag Purification Resin.

cOmplete^™ ULTRA Tablets, Mini, EDTA-free, EASYpack Protease Inhibitor Cocktail has been used as a component of

·       homogenization buffer for muscle samples for immunoblotting sample preparation[4]

·       radioimmunoprecipitation assay buffer (RIPA buffer) for protein extraction from brain samples for western blotting[5]

·       lysis buffer for the human embryonic kidney 293 (HEK293) lysate preparation for western blotting[6]

Features and Benefits

·       Protect your precious proteins.

Choose cOmplete ULTRA for even more efficient protease inhibition in eukaryotic, yeast, and bacterial cell lysates.

·       Why settle for less.

Benefit from 7 powerful protease inhibitors – more than in other EDTA-free inhibitor tablets.

·       Defend against a broad range of proteases.

Reliably inhibit serine, cysteine, and aspartic proteases.

·       Convenient and safe tablet format.

Eliminate weighing small amounts of individual inhibitors which might be hazardous. Simply drop a tablet from the blister into your buffers.

Components

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Physical form

Tablets in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution.

Preparation Note

Working concentration: 1 tablet per 10 ml extraction solution

Working solution: Handling

Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.

Preparation of Working Solution

One tablet is sufficient for the inhibition of proteolytic activity in 10 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 10 ml extraction buffer. The tablets can be added directly to the extraction medium.

Stock Solution (2x conc.)

Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:

• Add one cOmplete ULTRA Tablet, Mini, EDTA-free to 5 ml double-distilled water in a PE or PP tube.

• Vortex 3 min or until the tablet is completely dissolved.

• The stock solution is ready to use even if there are very fine parti­cles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.

• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.

• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.

Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.

Reconstitution

Soluble in aqueous buffers, or add directly to extraction media. For the stock solution, use water only.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

cOmplete is a trademark of Roche