RPMI 1640 Medium, with 25 mM HEPES, with L-Glutamine ยี่ห้อ HyClone

Supports cell growth in research and biomanufacturing cell culture processes.

• HyClone RPMI 1640 medium is extensively quality control tested to ensure lot-to-lot consistency.
• Medium efficacy and homogeneity of RPMI 1640 is tested with HyClone sera.

HyClone RPMI solution with L-glutamine is available with or without HEPES. RPMI 1640 formulations without L-glutamine as well as RPMI lyophilized powder are also available.

HyClone RPMI 1640 culture medium is hydrated using purified process water and is filtered through a 0.1 µm filter. HyClone growth media are manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities.

RPMI 1640 cell culture media composition

RPMI 1640 uses a sodium bicarbonate buffer system. The medium includes glucose and amino acids but does not include proteins, lipids, or growth factors. Therefore, nutrient supplementation is typically required using serum such as HyClone fetal bovine serum.

Cell lines for which RPMI 1640 cell culture media can be used

HyClone RPMI 1640 is a basal medium that was originally developed for long-term culture of peripheral blood lymphocytes. It is now used as a growth medium for a variety of mammalian cell types. Human leukocytes, hybridomas, and other mammalian cells can be grown in suspension or adherent culture with appropriate supplementation.

ParameterRPMI 1640 medium with HEPES, L-glutamine

AdditivesWith: HEPES, L-glutamine 

Shelf life18 months 

Storage2° to 8°C, away from light 

Sterilization0.1 µm sterile filtered 

FormatLiquid 

Pack size500 mL 

Order codeSH30255.01