GenElute™ HP Plasmid Maxiprep Kit

sufficient for 4 purifications

Synonym(s):

GenElute HP Plasmid Maxiprep Kit

NACRES:

NA.52

DESCRIPTION

General description

The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute^™ HP Plasmid Maxiprep Kit has been used to isolate plasmid DNA from Escherichia coli.

Features and Benefits

From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
Up to 1.2 mg of high-copy plasmid DNA
Flexibility of a vacuum or spin format
Contains fewer plastic components than other high speed kits, reducing the amount of waste
No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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GenElute™ HP Plasmid Maxiprep Kit , Prep. 4 ยี่ห้อ GenElute Sigma Aldrich