GenElute™ Bacterial Genomic DNA Kits, Prep. 350 ยี่ห้อ GenElute Sigma Aldrich
รหัสสินค้า : NA2120-1KT
| ราคา |
45,849.50 ฿ |
| จำนวนที่จะซื้อ | |
| ราคารวม | 45,849.50 ฿ |
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GenElute™ Bacterial Genomic DNA Kits
sufficient for 350 purifications
Synonym(s):
Bacterial Genomic DNA, Gen Elute
NACRES:
NA.55
DESCRIPTION
General description
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Application
The purified bacterial genomic DNA is ready for downstream applications such as:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
GenElute™ Bacterial Genomic DNA Kits has been used:
- to isolate DNA
- to prepare DNA from E. coli strain
- to extract genomic DNA
The purified bacterial genomic DNA is ready for downstream applications such as:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
Features and Benefits
- Starting material: Up to 1.5 mL of culture
- Expected yield: Up to 20 μg
- Elution volume: 400 μl
- Time required: 70 - 120 min
- A260/A280 ratio: 1.6 - 1.9
- No phenol, chloroform, or ethanol precipitation required
Principle
The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
**ราคาสินค้ารวมภาษีมูลค่าเพิ่ม 7% แล้ว**
