รหัสสินค้า : DNB600-20RXN

GenElute™ Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

DESCRIPTION

General description

Sigma′s GenElute^™ Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit provides a fast, reliable and simple procedures for isolating cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 4 mL. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 μL to 50 μL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

• Isolate all sizes of circulating DNA from plasma and serum samples
• Isolate viral and bacterial DNA
• Versatile plasma and serum input volumes (1 mL – 4 mL)
• Concentrate circulating DNA into a flexible elution volume ranging from (50 μL - 100 μL)
• Isolate inhibitor-free cell-free circulating DNA
• Purify high-quality DNA in 40-45 minutes
• Compatible with Streck Cell-Free DNA BCT Tubes

Application

• PCR
• qPCR
• Southern Blot
• Methylation-sensitive PCR
• CpG array
• Restriction enzyme digestions
• Viral Detection
• Bacterial Detection
• Microarray
• NGS

Unit Definition

Preps

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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