GenElute™ RNA/DNA/Protein Purification Plus Kit

DESCRIPTION

General description

Sigma′s GenElute^™ RNA/DNA/Protein Purification Plus Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Sigma′s GenElute^™ RNA/DNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications

Sequentially isolate and purify total RNA, genomic DNA and total proteins from a single sample
Ideal for small or difficult to obtain samples
No need to split the lysate, or to use phenol or precipitation methods
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
Rapid and efficient spin column procedure

Application

RNA

Bioanalyzer
Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
RT-PCR for both large mRNA and small RNA including miRNA
Northern blotting
RNase protection
Primer extension
Expression array assays
Next Generation Sequencing
microRNA Cloning
PCR-based virus detection
PCR-based viable bacteria detection
miRNA array
RNA-Seq
Nanostring
Fluidigm
Droplet/Digital PCR

Genomic DNA

PCR and Quantitative PCR
Sequencing
DNA Methylation Studies
Southern blotting
Microarrays
SNP analysis
PCR-based virus detection/identification
PCR-based bacteria detection/identification

Protein

SDS-PAGE analysis
Western blots
Mass Spectrometry

Features and Benefits

Complete column purification - The RNA, DNA and proteins are all column purified
Reduce variability - RNA, DNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
Isolate from small samples - Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures.
Rapid procedure - Isolate total RNA, genomic DNA and total proteins from a single sample in less than 30 minutes
Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
Isolate high quality RNA, DNA and proteins - The purified RNA, DNA and proteins are of the highest quality and can be used in a number of downstream applications

Unit Definition

Preps

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

**ราคาสินค้ารวมภาษีมูลค่าเพิ่ม 7% แล้ว**

GenElute™ RNA/DNA/Protein Purification Plus Kit, Prep. 50 ยี่ห้อ GenElute Sigma Aldrich