Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat, affinity isolated antibody ยี่ห้อ Sigma

PROPERTIES

biological source

goat

Quality Level

300

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

technique(s)

direct ELISA: 1:50,000

immunocytochemistry: suitable

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well

shipped in

dry ice

storage temp.

−20°C

DESCRIPTION

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Antibody is isolated from goat anti-mouse IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the Fc fragment of mouse IgG. Anti-Mouse IgG is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.

Immunogen

purified mouse IgG Fc fragment

Application

Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in

·       western blot

·       enzyme linked immunosorbent assay (ELISA) and

·       indirect immunoperoxidase assay (IIPA)

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.