Wizard(R) Plus SV Miniprep DNA Purif. Sys., 50 preps w/Adp. ยี่ห้อ Promega (Stroge. Temp +15C TO +30C)

1–20μg High-Quality Plasmid DNA from 1–10ml Cultures

• Process up to 20 minipreps in 45 minutes
• Choice of spin or vacuum purification formats
• No phenol extractions or precipitations required

Size 50 preps

Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® Plus SV Minipreps Start-Up Kits are available. Purchase a Wizard® Plus SV Minipreps DNA Purification System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.

The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector and can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

Item Part # Size Available Separately

Wizard® SV Minicolumns A129A 1 × 50/pk

Collection Tubes A130A 1 × 50/pk

Column Wash Solution (CWA) A131B 1 × 20ml View Product

Vacuum Adapters A1331 1 × 20 each

Alkaline Protease Solution A144A 1 × 550μl View Product

Neutralization Solution (NSB) A148B 1 × 30ml View Product

Cell Resuspension Solution (CRA) A711P 1 × 20ml View Product

Cell Lysis Solution (CLA) A712P 1 × 20ml View Product

Nuclease-Free Water P119E 1 × 13ml View Product

แหล่งที่มา : https://worldwide.promega.com/products/nucleic-acid-extraction/plasmid-purification/wizard-plus-sv-minipreps-dna-purification-systems/?catNum=A1340