pGEM(R)-T Easy Vector System II ยี่ห้อ Promega (Stroge. Temp LESS THAN -65C)

Convenience and Flexibility for Cloning PCR Products

• Three options for removing the insert with a single digest (BstZI, EcoRI or NotI )
• Ligation can be completed in 1 hour at room temperature
• Available with or without competent cells

Size 20

PCR Cloning with Blue/White Selection and Easy Insert Excision

The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The provided 2X Rapid Ligation Buffer allows reactions to be completed in 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation. 

T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by Blue/White Screening on indicator plates. The vector allows preparation of single-stranded DNA due to its f1 Origin of Replication.

The pGEM®-T Easy Vector Systems offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for BstZI, EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.

The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Easy Vector System I components.

Item Part # Size Concentration Available Separately

pGEM®-T Easy Vector A137A 1 × 1.2μg 50ng/μl

Control Insert DNA A363A 1 × 12μl

T4 DNA Ligase M180A 1 × 100u

2X Rapid Ligation Buffer C671A 1 × 200μl View Product

JM109 Competent Cells, High Efficiency L200A 6 × 200μl

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