AccessQuick™ RT-PCR System

Convenient RT-PCR in One Tube

• Mix contains Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer
• Separate AMV RT provided for important no-RT control reactions
• Simply combine AccessQuick™ Master Mix with AMV RT, RNA template, primers and water for efficient RT-PCR

Master Mix for Single-Tube RT-PCR

The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases convenience by combining Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer in a single master mix.  AMV RT enzyme is provided separately to allow important no-RT control reactions. The master mix is simply added to RNA templates in reaction vials, followed by AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.

• Amplify zeptomole (10^–21mol) levels of RNA.
• Set up reactions in a single tube, place in the thermal cycler and come back later for results—no additions between the reverse transcription and DNA amplification steps.
• System components are stable over many freeze-thaw cycles.

Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

ItemPart #SizeAvailable Separately

AccessQuick™ Master Mix (2X)A170C1 × 12.5ml

AMV Reverse TranscriptaseA261C1 × 2,500u

Nuclease-Free WaterP119C1 × 25mlView Product

แหล่งที่มา : https://worldwide.promega.com/products/pcr/rt-pcr/accessquick-rt-pcr-system/?catNum=A1703