Reverse Transcription System

Convenient System for Reverse Transcription Using AMV Reverse Transcriptase

The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)₁₅ and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications. A polyadenylated 1.2kb RNA transcript is provided as a control template for cDNA synthesis.

• Speed: Efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes.
• Convenience: PCR-compatible components are provided in optimized volumes for 100 reactions.
• Positive Controls: A polyadenylated RNA transcript is provided to help troubleshoot RT-PCR parameters.
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ItemPart #SizeConcentrationAvailable Separately

MgCl₂A351H1 × 1.2ml25mMView Product

Reverse Transcription 10X BufferA356A1 × 1.4mlView Product

Oligo(dT)₁₅ PrimerC110B1 × 50μg500μg/mlView Productd

NTP MixC114B1 × 320μl10mMView Product

Random PrimersC118B1 × 50μgView Product

1.2kb Kanamycin Positive Control RNAC138A1 × 5μg0.5mg/mlView Product

AMV Reverse Transcriptase (HC)M900B1 × 1,500uView Product

Recombinant RNasin® Ribonuclease InhibitorN251A1 × 2,500u40u/μlView Product

Nuclease-Free WaterP119E1 × 13mlView Product

แหล่งที่มา : https://worldwide.promega.com/products/pcr/rt-pcr/reverse-transcription-system/?catNum=A3500