pGEM®-T Vector Systems

Convenience for Cloning PCR Products

• Insert excision with a BstZI single digest
• Ligation can be completed in 1 hour at room temperature
• Available with or without competent cells

Efficient T-Vector Cloning with Blue/White Selection

The pGEM®-T Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Vector is ready to use in ligation reactions, prepared by cutting the pGEM®-5Zf(+) Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for ligation of PCR products with A overhangs.

ItemPart #SizeAvailable Separately

pGEM®-T VectorA362A1 × 1.2μg

Control Insert DNAA363A1 × 12μl

2X Rapid Ligation BufferC671A1 × 200μlView Product

JM109 Competent Cells, High EfficiencyL200A6 × 200μl

T4 DNA LigaseM180A1 × 100u

แหล่งที่มา : https://worldwide.promega.com/products/pcr/pcr-cloning/pgem-t-vector-systems/?catNum=A3610