Ammonium persulfate (APS), for molecular biology, suitable for electrophoresis, ≥98% ยี่ห้อ Millipore

PROPERTIES

grade

for molecular biology

Quality Level

200

vapor density

7.9 (vs air)

Assay

≥98%

form

powder

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

anion traces

chloride (Cl-): <10 ppm

cation traces

Fe: <10 ppm

heavy metals (as Pb): <50 ppm

foreign activity

DNAse, none detected

Protease, none detected

RNAse, none detected

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

DESCRIPTION

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size.[1] APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.[2]

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.[3][4][5]

Catalyst for acrylamide gel polymerization.

Analysis Note

Tested for use in acrylamide polymerization.