Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in goat, affinity isolated antibody buffered aqueous solution ยี่ห้อ Sigma

PROPERTIES

biological source

goat

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

human, rat

technique(s)

direct ELISA: 1:40,000

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane)

shipped in

dry ice

storage temp.

−20°C

DESCRIPTION

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense.[1] It is a glycoprotein. IgG is a major class of immunoglobulin.[2] Mice possess five classes of immunoglobulins- IgM, IgG, IgA, IgD and IgE.[2] Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.[3]

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG.

Immunogen

purified mouse IgG, Fab fragment

Application

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.

Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:

·       enzyme-linked immunosorbent assay (ELISA)

·       immunohistochemical studies

·       dot or immunoblotting

Goat anti-mouse IgG (Fab specific)-peroxidase antibody can be used for western blotting applications. The product can also be used for direct ELISA (1:40,000) and immunohistochemistry (at 1:150, using formalin-fixed, paraffin-embedded sections).

HRP conjugated goat anti-mouse IgG Fab specific was used as the secondary antibody for western blot analysis of bacterial samples of s. equi cells to detect fibrinogen-binding proteins.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.[2] IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.[3]

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Adsorbed with human IgG and rat serum proteins to reduce background staining with human or rat samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.