ImProm-II™ Reverse Transcription System

Reverse Transcribe RNA Templates Up to 8.9kb

Complete System for First-Strand cDNA Synthesis of Long or Rare Messages

The ImProm-II™ Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of the ImProm-II™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and powerful ImProm-II™ Reverse Transcriptase provided in the ImProm-II™ System together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1–20μl) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.

Advantages

• Amenable to Full-Length RT-PCR: Reverse transcribe long RNA templates up to 8.9kb.
• Microarray-Compatible: May be used to incorporate regular, Cy®3-modified, Cy®5-modified and amino-allyl-modified nucleotides.
• Easy to Use: System provides all reagents necessary for efficient reverse transcription.
• Scalable and Flexible: 1–20μl of the initial reverse transcription reaction may be used in subsequent PCR, and the optimized buffer allows coupled RT-PCR.
• RT Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl₂ Solution also is included.
• Versatile: Use with your thermostable DNA polymerase of choice.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

ItemPart #SizeConcentrationAvailable Separately

Upstream Control PrimerA109B1 × 100μl15μ

MDownstream Control PrimerA110B1 × 100μl15μ

MMgCl₂A351H1 × 1.2ml25mMView Product

Oligo(dT)₁₅ PrimerC110B1 × 50μg500μg/mlView Productd

NTP MixC114B1 × 320μl10mMView Product

Random PrimersC118B1 × 50μgView Product

1.2kb Kanamycin Positive Control RNAC138A1 × 5μg0.5mg/mlView Product

ImProm-II™ 5X Reaction BufferM289A1 × 600μl

ImProm-II™ Reverse TranscriptaseM314A1 × 100μl

Recombinant RNasin® Ribonuclease InhibitorN251A1 × 2,500u40u/μlView Product

Nuclease-Free WaterP119A2 × 1,250μlView Product

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