Proteinase K (PK) Solution

Stable at Room Temperature and Easy to Use

• Active over pH range 4.3–12.0 in 0.5% SDS or 1% Triton® X-100
• Retains >80% activity at temperatures up to 60°C
• No resuspension or thawing before use
• Cat.# MC5005, MC5008 supplied at a concentration of 20mg/ml

Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples. It has been purified to remove RNase and DNase activities. The stability of Proteinase K in urea and SDS and its ability to digest native proteins make it useful for a variety of applications including preparation of chromosomal DNA for pulsed-field gel electrophoresis, protein fingerprinting and removal of nucleases from preparations of DNA and RNA. A typical working concentration for Proteinase K is 50–100μg/ml.

Formulation: Proteinase K (PK) Solution is supplied at a concentration of 20mg/ml in 10mM Tris-HCl (pH 7.5), 1mM calcium chloride and 50% glycerol.

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References:

1. Ebeling, W. et al. (1974) Eur. J. Biochem. 47, 91–7.
2. Schwartz, D.C. and Cantor, C.R. (1984) Cell 37, 67–75.
3. Cleveland, D.W. et al. (1977) J. Biol. Chem. 252, 1102–6.
4. Hames, B.D. (1981) In: Gel Electrophoresis of Proteins: A Practical Approach, B.D. Hames and D. Rickwood, eds., IRL Press, Oxford, 219.
5. Herrmann, B.G. and Frischauf, A.M. (1987) Meth. Enzymol. 152, 180–3.
6. Lee, J.J. and Costlow, N.A. (1987) Meth. Enzymol. 152, 633–48.
7. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Vol. 3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
8. Sweeney, P.J. and Walker, J.M. (1993) Enzymes of molecular biology. In: Methods in Molecular Biology, Vol. 16, M.M. Burrell, ed., Humana Press, Inc., Totowa, NJ, 305.

ItemPart #Size

Proteinase K (PK) SolutionA50511 × 23ml

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