qPCR Kits: GoTaq® qPCR and RT-qPCR Systems

Convenient Master Mixes for Real-Time qPCR and RT-qPCR

• Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR
• Compatible with both fast and standard cycling methods
• Resistant to a wide range of PCR inhibitors

Simple, Sensitive, Dye-Based qPCR and RT-qPCR

The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I.

Rapid hot-start activation and processive enzymes make the GoTaq® Systems compatible with most real-time PCR instruments using standard or fast cycling. The 1-Step and 2-Step RT-qPCR Systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR.

ItemPart #SizeConcentrationAvailable Separately

MgCl₂A351B1 × 750μl25mMView Product

GoScript™ 5X Reaction BufferA500B1 × 300μlView Product

GoScript™ Reverse TranscriptaseA501B1 × 50μl

GoTaq® qPCR Master Mix, 2XA600A5 × 1ml

Oligo(dT)₁₅ PrimerC110B1 × 50μg500μg/mlView Product

PCR Nucleotide MixC114G1 × 200μl10mMView Product

Random PrimersC118B1 × 50μgView Product

CXR Reference DyeC541A1 × 100μlView Product

Recombinant RNasin® Ribonuclease InhibitorN251A1 × 2,500u40u/μlView Product

Nuclease-Free WaterP119E2 × 13mlView Product

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