Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat, affinity isolated antibody buffered aqueous solution ยี่ห้อ Sigma

PROPERTIES

biological source

goat

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

Show Less

DESCRIPTION

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is immunospecific for rabbit IgG by immunoelectrophoresis against normal serum and rabbit IgG, prior to conjugation.

Immunogen

purified rabbit IgG

Application

C33A cell lysates were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Protein aliquots from colonic mucosal extracts (μg) were anlayzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:5000 dilution in TBSt for 1 hr at room temperature.

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody has been used for western blot (1:6,000), and ELISA assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.