Wizard® Plus Maxipreps DNA Purification System

300µg–1mg Plasmid DNA from 100–500ml Cultures

• 3-hour protocol
• Resin-based batch-binding and column-washing method
• No organic extractions

The Wizard® Plus Maxipreps DNA Purification System provides a rapid resin-based batch column method for purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. This system typically yields up to 500μg of high-copy-number plasmid DNA (200–20,000bp) from a 100–500ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

ItemPart #SizeAvailable Separately

Cell Resuspension Solution

A711C1 × 150mlView Product

Cell Lysis Solution

A712D1 × 150mlView Product

Neutralization Solution

A713C2 × 150mlView Product

Maxi/Megapreps Filtering System

A742D2 × 5 packView Product

Wizard® Maxipreps DNA Purification Resin

A769A1 × 100mlView Product

Column Wash Solution

A810C1 × 125mlView Product

แหล่งที่มา : https://worldwide.promega.com/products/nucleic-acid-extraction/plasmid-purification/wizard-plus-maxipreps-dna-purification-system/?catNum=A7270