Wizard(R) Plus Megapreps DNA Purification System, 5 preps ยี่ห้อ Promega (Stroge. Temp +15C TO +30C)
รหัสสินค้า : A7300
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Wizard® Plus Megapreps DNA Purification System
More than 1mg Plasmid from 1L Cultures
- 3-hour protocol
- Resin-based batch column method
- No organic extractions
The Wizard® Plus Megapreps DNA Purification System provides a rapid method for large-scale purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. Plasmid DNA yields more than one milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Wizard® Plus Megapreps requires the use of a vacuum source and either a lab-assembled or commercial manifold, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) for up to 20 samples or the Vac-Man® Jr. Manifold (Cat.# A7660) for 1 or 2 samples.
ItemPart #SizeAvailable Separately
Cell Resuspension Solution
A711C1 × 150mlView Product
Cell Lysis Solution
A712D1 × 150mlView Product
Neutralization Solution
A713C2 × 150mlView Product
Maxi/Megapreps Filtering System
A742D1 × 5 packView Product
Wizard® Megapreps DNA Purification Resin
A768A1 × 100mlView Product
Column Wash Solution
A810C1 × 125mlView Product
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