Wizard® Plus Minipreps DNA Purification System

Plasmid Purification from 1–10ml Cultures

• 15-minute protocol
• Resin-based batch column method
• No organic extraction or ethanol precipitation

The Wizard® Plus Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA in 15 minutes or less, with no organic extractions or ethanol precipitations. Minipreps may be processed individually or in multiples with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard® Minicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor, such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511). Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing.

The Wizard® Minipreps DNA Purification Resin is used in the isolation and preparation of plasmid DNA in conjunction with the Wizard® Plus Minipreps DNA Purification Systems. The resin is available with the systems and as a standalone product.

This product requires plungers (not provided) for use with the purification protocol without a vacuum manifold.

Each A7100 system contains all reagents required for either 50 standard minipreps from 1–3ml of culture, 30 standard minipreps from 3–5ml of culture or 25 standard minipreps from 5–10ml of culture (using EndA– strains).

ItemPart #SizeAvailable Separately

Cell Resuspension Solution

A711E1 × 50mlView Product

Cell Lysis Solution

A712E1 × 50mlView Product

Neutralization Solution

A713J1 × 100mlView Product

Wizard® Minicolumns

A72111 × 250 each

Wizard® Minipreps DNA Purification Resin

A767G1 × 250ml

Syringe Barrels, 3cc

A809B5 × 50 pack

Column Wash Solution (Modified)

A810E1 × 230mlView Product

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