Wizard® Plus Midipreps DNA Purification System

Up to 200µg Plasmid DNA from 10–100ml Cultures

• Rapid, 90-minute protocol
• Resin-based batch column method
• No organic extractions

The Wizard® Plus Midipreps DNA Purification System isolates plasmid DNA in 90 minutes or less, yielding up to 200μg of high-quality DNA with no organic extractions or ethanol precipitations. Multiple midipreps can be easily processed at one time with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard® Midicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing or restriction digestion without further manipulation and also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

Plasmid DNA is of comparable quantity and quality to that obtained using time-consuming cesium chloride:ethidium bromide gradient techniques. The purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing.

Wizard® Plus Midipreps requires the use of a vacuum source and either a lab-assembled or commercial manifold, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) for up to 20 samples or the Vac-Man® Jr. Manifold (Cat.# A7660) for 1 or 2 samples.

ItemPart #SizeAvailable Separately

Cell Resuspension Solution (CRA)A711F1 × 75mlView Product

Cell Lysis Solution (CLA)A712F1 × 75mlView Product

Neutralization Solution (NSA)A71311 × 150ml

Wizard® Midipreps DNA Purification ResinA770A1 × 250mlView Product

Column Wash Solution (CWB)A81021 × 125ml

Column Wash Solution (CWB)A810F1 × 230mlView Product

Wizard® MidicolumnsA765A1 × 25 eachView Product

แหล่งที่มา : https://worldwide.promega.com/products/nucleic-acid-extraction/plasmid-purification/wizard-plus-midipreps-dna-purification-system/?catNum=A7640