Acrylamide, suitable for electrophoresis, ≥99% ยี่ห้อ Sigma

PROPERTIES

vapor density

2.45 (vs air)

Quality Level

200

vapor pressure

0.03 mmHg ( 40 °C)

Assay

≥99%

form

crystals

technique(s)

electrophoresis: suitable

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

suitability

suitable for electrophoresis

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

DESCRIPTION

General description

Acrylamide acts as a quencher of tryptophan fluorescence.[1] Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.[1] Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.[2]

Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide.[3] This highly reactive organic compound has the ability to polymerize to form polyacrylamide.[4]

Application

Acrylamide has been used:

·       for the synthesis of hydrogels[5][6]

·       for the preparation of polyacrylamide gel[7][8]

·       to agglomerate vimentin and keratin intermediate filament network[9]

Acrylamide has been used:

·       to agglomerate the vimentin and keratin intermediate filament network of K562cells[9]

·       in the preparation and characterization of soft culture substrate[8]

·       in hydrogel synthesis[10]

·       for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis[11]