Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in goat, affinity isolated antibody buffered aqueous solution ยี่ห้อ Sigma

PROPERTIES

biological source

goat

Quality Level

300

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:60,000

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

western blot: 1:80,000-1:160,000

shipped in

dry ice

storage temp.

−20°C

DESCRIPTION

General description

Immunoglobulin G (IgG) is a glycoprotein antibody.[1] IgG belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond.[2] Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE.[1] Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.[3][4]

Specificity

Specificity of the Anti-Mouse IgG (Fab Specific)- Peroxidase is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, single precipitation arcs are observed against normal mouse serum, mouse IgG and the Fab fragment of mouse IgG, while no reaction is observed against the Fc fragment of mouse IgG or human IgG. Binds all mouse IgGs.

Immunogen

Purified mouse IgG Fab fragment

Application

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.

Anti-Mouse IgG (Fab specific)-Peroxidase antibody has been used in immunofluorescence, ELISA (enzyme-linked immunosorbent assay) and immunoblotting.

Goat Anti-Mouse IgG (Fab specific)-Peroxidase antibody can be used for western blotting assays. The product can also be used for direct ELISA (1:60,000) and IHC (1:150) applications.

Bovine CVEC and human epidermoid carcinoma A431 cell lysates were analyzed by western blot using HRP-conjugated goat anti-mouse IgG Fab specific as the secondary antibody at a 1:3000 dilution.

Biochem/physiol Actions

Immunoglobulin G (IgG) regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases.[2] IgG1 regulates complement fixation, opsonization and antibody dependent cell mediated cytotoxicity in mice.[3] IgG participates in hypersensitivity type II and type III.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with human samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Storage and Stability

Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.