Anti-Goat IgG Antibody, HRP conjugate; produced in rabbit; Purified rabbit IgG conjugated to horseradish peroxidase in buffer containing 0.01 M Sodium Phosphate, ยี่ห้อ Sigma

PROPERTIES

biological source

rabbit

Quality Level

100

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

goat

manufacturer/tradename

Chemicon^®

concentration

1 mg/mL (after reconstitution)

technique(s)

ELISA: suitable

immunohistochemistry: suitable

western blot: suitable

isotype

IgG

shipped in

wet ice

DESCRIPTION

General description

Affinity purified rabbit anti-goat IgG, Peroxidase conjugated.

Specificity

Specific for goat IgG, heavy and light chain.

Application

ELISA and Western Blots:

1:5,000-1:100,000 dilution can be used.

Immunohistochemistry:

1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.

Rabbit anti-Goat IgG Antibody, HRP conjugate is an antibody against Goat IgG for use in ELISA, IH & WB.

Research Category

Secondary & Control Antibodies

Research Sub Category

Whole Immunoglobulin Secondary Antibodies

Physical form

Immunoaffinity chromatography using antigen coupled to sepharose beads.

Purified Rabbit IgG conjugated to horseradish peroxidase in buffer containing 0.01M PBS, pH 7.1, with 15mg/mL BSA. Lyophilized.

Storage and Stability

Lyophilized product is stable at 2-8°C for up to 12 months from date of shipment.

After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

SAFETY INFORMATION

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable