Goat Anti-Mouse IgG & IgM Antibody, HRP Conjugate ยี่ห้อ Sigma-Aldrich

PROPERTIES

biological

source

goat

Quality

Level

100

conjugate

peroxidase conjugate

antibody

form

F(ab′)₂ fragment

of affinity isolated antibody

antibody

product type

secondary antibodies

clone

polyclonal

species

reactivity

mouse

manufacturer/tradename

Chemicon^®

concentration

0.8 mg/mL

technique(s)

ELISA: suitable

western blot: suitable

shipped

in

wet ice

target

post-translational modification

unmodified

DESCRIPTION

General description

Immunoglobulin G (IgG) is the most abundant immunoglobulin in the human serum. IgM constitutes 5-10% of total serum immunoglobulins. These immunoglobulins comprise four polypeptide chains with two heavy (H) chains and two light (L) chains. These polypeptide chains are inter-linked by disulfide bonds. IgM is a highly conserved antibody in vertebrates. IgM exists as a pentamer. Both IgG and IgM are secreted by B cells.

Application

Goat Anti-Mouse IgG & IgM Antibody, HRP conjugate has been used in western blotting[1](1:400) and immunofluorescence (1:500)[2]

Biochem/physiol Actions

IgG facilitates antibody-dependent cell-mediated cytotoxicity (ADCC). It also aids in the activation of the classical pathway of the complement system. IgG neutralizes virus particles and toxins. IgM, an antibody that is expressed early during an immune response, aids in engulfing apoptotic cells. It exhibits polyreactive and autoreactive functionality. IgM induces tissue homeostasis by mediating the clearance of tissue-based molecules. Lower levels of IgM in serum are associated with increased autoimmune response and a higher risk of developing infections.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable