Goat Anti-Rabbit IgG Antibody, Biotin-SP Conjugate ยี่ห้อ Sigma-Aldrich

PROPERTIES

biological source

goat

Quality Level

100

conjugate

biotin conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon^®

technique(s)

ELISA: suitable

western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

DESCRIPTION

General description

Affinity purified goat anti-rabbit IgG, Biotin conjugated.

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

EIA: 1:50,000-1:100,000

Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000

Flow cytometry: 1:200-1:1,000

Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000

Optimal working dilutions must be determined by end user.

Goat anti-Rabbit IgG Antibody, biotin-SP conjugate detects level of Rabbit IgG & has been published & validated for use in ELISA & WB.

Research Category

Secondary & Control Antibodies

Research Sub Category

Whole Immunoglobulin Secondary Antibodies

Physical form

ImmunoAffinity Purified

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

For research use only; not for use as a diagnostic.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.