Anti-Mouse IgG Antibody, (H+L) HRP conjugate; produced in goat; Purified Goat IgG in buffer containing liquid in PBS, pH 7.1 with 15 mg/ mL BSA and 0.01% thimerosal. ยี่ห้อ Sigma

PROPERTIES

biological source

goat

Quality Level

100

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon^®

concentration

1 mg/mL

technique(s)

ELISA: suitable

immunocytochemistry: suitable

immunohistochemistry: suitable

western blot: suitable

isotype

IgG

shipped in

wet ice

DESCRIPTION

General description

The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer.

Specificity

Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.

Application

ELISA:

1:3,000 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

Immunohistochemistry/immunocytochemistry:

1:200-1:500 dilution can be used (Bullock, G.R., 1982; Javois, L.C., 1994).

Optimal working dilutions must be determined by the end user.

Research Category

Secondary & Control Antibodies

Research Sub Category

Whole Immunoglobulin Secondary Antibodies

This Goat anti-Mouse IgG Antibody, (H+L) HRP conjugate is validated for use in ELISA, IC, IH, WB for the detection of Mouse IgG.

Quality

Western Blot, chromogenic:

1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

Western Blot, chemiluminescent:

1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).

Physical form

Affinity purified

Purified Goat IgG conjugated to horseradish peroxidase in buffer containing liquid in 0.01M PBS, pH 7.1 with 15mg/mL BSA and 0.1% Kathon.

Storage and Stability

The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format. DO NOT FREEZE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.