PowerSeq® CRM Nested System, Custom

Produce MPS Libraries Ready for Mitochondrial Control Region Analysis

• Massively parallel sequencing of mitochondrial HVI, HVII and HVIII control regions in a single reaction
• Nested amplification protocol greatly reduces number of steps and time required for library preparation
• Small amplicons ranging from 144–237bp for better results with compromised samples

Massively parallel sequencing (MPS) allows laboratories access to mitochondrial DNA (mtDNA) analysis using a more sensitive and high-throughput workflow compared to traditional sequencing methods. Increased mixture deconvolution and heteroplasmy resolution are achieved by deep sequencing coverage and digital read counts. Additionally, the use of small amplicons to sequence the mitochondrial control region improves sequencing results from degraded samples.

The PowerSeq® CRM Nested System, Custom, generates 10 small amplicons covering the control region of the mitochondrial genome in one multiplex. The targeted regions for amplification are designed to be in a range of 144–237bp to ensure optimal results from degraded samples. The system uses a nested amplification protocol that greatly reduces the number of steps and time required to produce libraries ready for sequencing. The protocol consists of a single PCR step to both amplify the target amplicons and incorporate indexed sequencing adapters.

Sequencing data can be analyzed using GeneMarker HTS Software (SoftGenetics, LLC).

ItemPart #SizeConcentrationAvailable Separately

PowerSeq® CRM Nested 10X Primer Pair Mix

AX579A1 × 250μl

PowerSeq® Nested System Index Primer 1 D701

AX580A1 × 75μl

PowerSeq® Nested System Index Primer 1 D702

AX582A1 × 75μl

PowerSeq® Nested System Index Primer 1 D703

AX583A1 × 75μl

PowerSeq® Nested System Index Primer 1 D704

AX584A1 × 75μl

PowerSeq® Nested System Index Primer 1 D705

AX585A1 × 75μl

PowerSeq® Nested System Index Primer 1 D706

AX586A1 × 75μl

PowerSeq® Nested System Index Primer 1 D707

AX587A1 × 75μl

PowerSeq® Nested System Index Primer 1 D708

AX588A1 × 75μl

PowerSeq® Nested System Index Primer 1 D709

AX589A1 × 75μlPowerSeq® Nested System Index Primer 1 D710

AX590A1 × 75μl

PowerSeq® Nested System Index Primer 1 D711

AX591A1 × 75μl

PowerSeq® Nested System Index Primer 1 D712

AX592A1 × 75μl

PowerSeq® Nested System Index Primer 2 D501

AX593A1 × 75μl

PowerSeq® Nested System Index Primer 2 D502

AX594A1 × 75μl

PowerSeq® Nested System Index Primer 2 D503

AX595A1 × 75μl

PowerSeq® Nested System Index Primer 2 D504

AX596A1 × 75μl

PowerSeq® Nested System Index Primer 2 D505

AX597A1 × 75μl

PowerSeq® Nested System Index Primer 2 D506

AX598A1 × 75μl

PowerSeq® Nested System Index Primer 2 D507

AX599A1 × 75μl

PowerSeq® Nested System Index Primer 2 D508

AX600A1 × 75μl

2800M Control DNA

DD710A1 × 25μl

10ng/μlView ProductPowerSeq® 5X Master Mix

DP116A1 × 500μl

Water, Amplification Grade

DW099A2 × 1,250μlView Product

Proteinase K

V302A1 × 10mgView Product

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