Bromophenol Blue sodium salt, for molecular biology, for electrophoresis ยี่ห้อ Sigma

PROPERTIES

grade

for molecular biology

Quality Level

200

form

powder

technique(s)

electrophoresis: suitable

visual transition interval

3.0-4.6, yellow to blue

ε (extinction coefficient)

≥150000 at 597-603 nm in H₂O

≥17000 at 250-256 nm in H₂O

≥23000 at 306-312 nm in H₂O

application(s)

diagnostic assay manufacturing

hematology

histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

DESCRIPTION

General description

Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.[1]

Application

Bromophenol Blue sodium salt has been used in the buffer solution to differentiate dead and living cells.[2] It has also been used as a constituent in gel loading buffer.[3][4][5]

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

SAFETY INFORMATION

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable