Spin Columns (including collection & wash tubes), 10ea ยี่ห้อ Promega (Stroge. Temp +15C TO +30C)
รหัสสินค้า : C1281
ราคา |
0.00 ฿ |
จำนวนที่จะซื้อ | |
ราคารวม | 0.00 ฿ |
Universal RiboClone™ cDNA Synthesis System
- Reagents to synthesize double-stranded cDNA from up to 40µg of mRNA
- Oligo(dT)15 Primer and Random Primers included for choice of priming methods
- Materials provided for 10 adaptor ligation reactions
The Universal RiboClone™ cDNA Synthesis System is used for double-stranded cDNA synthesis from RNA. The system also includes materials for ligation of the cDNA into a suitable vector. First-strand synthesis is driven by Avian Myeloblastosis Virus (AMV) Reverse Transcriptase and either Random Primers or Oligo(dT)15 Primer. This is followed directly by second-strand synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to create blunt ends, the double-stranded cDNA molecules are prepared for cloning by size fractionation and addition of EcoRI Adaptors. The resulting cDNA preparation is ready for cloning into a suitable vector.
ItemPart #SizeConcentrationAvailable Separately
Sodium PyrophosphateC113A1 × 50μl40mM
First Strand 5X BufferC121A1 × 200μl
T4 DNA Ligase 10X BufferC126A1 × 500μlView Product
Spin ColumnsC12811 × 10 eachView Product
EcoRI AdaptorsC129A1 × 150pmolView Product
Kinase 10X BufferC131A1 × 500μlView Product
ATPC134A1 × 50μl
1.2kb Kanamycin Positive Control RNAC138B1 × 10μg0.5mg/mlView Product
Oligo(dT)15 PrimerC144A1 × 20μg500μg/mlView Product
Random PrimersC146A1 × 20μgView Product
Second Strand 2.5X Buffer, AMVC630A1 × 800μl
T4 DNA LigaseM180A1 × 100u
DNA Polymerase IM205A1 × 500u
T4 Polynucleotide KinaseM410A1 × 100uView Product
T4 DNA PolymeraseM421A1 × 100u
RNase HM428B1 × 20u
AMV Reverse Transcriptase (HC)M900A1 × 600uView Product
Recombinant RNasin® Ribonuclease InhibitorN251E1 × 1,000u
Nuclease-Free WaterP119A1 × 1,250μlView Product
Acetylated BSAR946A1 × 400μlView Product
Sephacryl® S-400V318A1 × 10mlView Product
แหล่งที่มา : https://worldwide.promega.com/products/pcr/rt-pcr/universal-riboclone-cdna-synthesis-system/?catNum=C4360
สินค้าไม่เพียงพอ
สินค้าหมด