Universal RiboClone™ cDNA Synthesis System

• Reagents to synthesize double-stranded cDNA from up to 40µg of mRNA
• Oligo(dT)₁₅ Primer and Random Primers included for choice of priming methods
• Materials provided for 10 adaptor ligation reactions

The Universal RiboClone™ cDNA Synthesis System is used for double-stranded cDNA synthesis from RNA. The system also includes materials for ligation of the cDNA into a suitable vector. First-strand synthesis is driven by Avian Myeloblastosis Virus (AMV) Reverse Transcriptase and either Random Primers or Oligo(dT)₁₅ Primer. This is followed directly by second-strand synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to create blunt ends, the double-stranded cDNA molecules are prepared for cloning by size fractionation and addition of EcoRI Adaptors. The resulting cDNA preparation is ready for cloning into a suitable vector.

ItemPart #SizeConcentrationAvailable Separately

Sodium PyrophosphateC113A1 × 50μl40mM

First Strand 5X BufferC121A1 × 200μl

T4 DNA Ligase 10X BufferC126A1 × 500μlView Product

Spin ColumnsC12811 × 10 eachView Product

EcoRI AdaptorsC129A1 × 150pmolView Product

Kinase 10X BufferC131A1 × 500μlView Product

ATPC134A1 × 50μl

1.2kb Kanamycin Positive Control RNAC138B1 × 10μg0.5mg/mlView Product

Oligo(dT)₁₅ PrimerC144A1 × 20μg500μg/mlView Product

Random PrimersC146A1 × 20μgView Product

Second Strand 2.5X Buffer, AMVC630A1 × 800μl

T4 DNA LigaseM180A1 × 100u

DNA Polymerase IM205A1 × 500u

T4 Polynucleotide Kinase

M410A1 × 100uView Product

T4 DNA PolymeraseM421A1 × 100u

RNase HM428B1 × 20u

AMV Reverse Transcriptase (HC)M900A1 × 600uView Product

Recombinant RNasin® Ribonuclease InhibitorN251E1 × 1,000u

Nuclease-Free WaterP119A1 × 1,250μlView Product

Acetylated BSAR946A1 × 400μlView Product

Sephacryl® S-400V318A1 × 10mlView Product

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