CellASIC ONIX2 Microfluidic System ยี่ห้อ Sigma

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General description

CellASIC^® ONIX2 Microfluidic System is an automated platform for precise manipulation of multiple key cell culture parameters, enabling measurement of cellular responses to pre-programmed media, temperature, and gas environment changes. It provides detailed information about live cell processes with ultra-high quality images and complete environment control. The system utilizes high quality, optically clear microfluidic plates and intuitive software, while integrating with a broad range of inverted microscopes to allow continuous, high magnification observation of live cells—as they react to their environment in time.

Includes:

·      Controller System: Small footprint, integrated microincubator controller maintains fluid movement, reagent additions, temperature, and gas conditions.

·      Microfluidic Plates: Application specific plates bring new cell culture capabilities for live cell imaging.

·      Manifold: Low-profile, high quality, high optical clarity culture chambers and manifold, mount easily on an inverted microscope—without the need for a bulky environmental chamber.

·      Software: Intuitive software enables quick and easy set-up of detailed protocols for truly automated hands-free cell culture.

Controller System:

·      Automatically control flow rates, gas and temperature shifts, standing gradients, nutrient/drug additions, and media changes.

·      Eight critical cell culture parameters can be controlled by the system.

·      Temperature, oxygen level, growth factors, humidity, pH, osmolality, glucose, ECM and adhesion.

Advantages of the CellASIC^® ONIX2 microfluidic plates:

·      High resolution imaging through optical glass bottom and chamber structures.

·      Ability to run multiple independent experiments simultaneously.

·      Continuous access to nutrients and ongoing removal of waste, promoting optimal cell health.

Manifold Advantages:

·      Manifold seals to the microfluidic plate via “one-touch” vacuum mechanism.

·      Pressure-driven flow of liquids to the cell chamber provides high precision even at very small volumes.

·      No contact between the flow system and the biological solutions (on the plate) prevents contamination.

·      Low-profile manifold is easy to adapt to any inverted microscope stage.

The software offers:

·      Clearly defined protocols with all experimental parameters saved in one file.

·      Convenience and reproducibility.

·      Consistency and reliability.

·      Error checking, self test, and data logging all make for better troubleshooting and serviceability.

Application

·      Yeast single cell response.

·      Bacterial single cell response.

·      Bacterial biofilm dynamics.

·      Cell response to hypoxic environments.

·      Cell response to changing media conditions, drugs, and other stimulants.

·      Host-pathogen interactions.

·      Chemotaxis/migration in response to chemogradient.

·      Setting optimal culture parameters for difficult cell types or long term experiments.

·      Microscopy of 3D cell culture.

Legal Information

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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