Human Fc gamma RIIA / CD32a (H167) Protein, His Tag (SPR & BLI & MALS verified) ยี่ห้อ Acrobiosystems

รหัสสินค้า : CD1-H5223-50ug

Synonym

• CD32a, FCGR2A, CD32, FCG2 , FCGR2A1, IGFR2

Source

• Human CD32a (H167), His Tag (CD1-H5223) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1).

Molecular Characterization

• This protein carries a polyhistidine tag at the C-terminus.
• The protein has a calculated MW of 21.5 kDa. The protein migrates as 29-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

• Less than 1.0 EU per μg by the LAL method.

Purity

• >95% as determined by SDS-PAGE.
• >90% as determined by SEC-MALS.

Formulation

• Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Contact us for customized product form or formulation.

Reconstitution

• Please see Certificate of Analysis for specific instructions.
• For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

• For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Please avoid repeated freeze-thaw cycles.
• This product is stable after storage at:

1. -20°C to -70°C for 24 months in lyophilized state;
2. -70^°C for 12 months under sterile conditions after reconstitution.

SDS-PAGE

Human CD32a (H167), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

The purity of Human CD32a (H167), His Tag (Cat. No. CD1-H5223) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Report

Bioactivity-SPR

Immobilized Human CD32a (H167), His Tag (SPR & BLI verified) (Cat. No. CD1-H5223) on CM5 Chip via anti-His antibody, can bind  Rituximab with an affinity constant of 1.45 μM as determined in a SPR assay (Biacore T200) (QC tested).

Bioactivity-BLI

Loaded Human CD32a (H167), His Tag (SPR & BLI verified) (Cat. No. CD1-H5223) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

SEC-MALS

The purity of Human CD32a (H167), His Tag (Cat. No. CD1-H5223) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Rituximab immobilized on CM5 Chip can bind Human CD32a (H167), His Tag (Cat. No. CD1-H5223) with an affinity constant of 0.882 μM as determined in SPR assay (Biacore 8K) (Routinely tested).