Human Fc gamma RIIB/C (CD32b/c) Protein, His Tag (SPR & BLI & MALS verified)

Synonym

• FCGR2B, C, CD32b, c, FcRII-b, c, Fc-gamma RII-b, c, Fc-gamma-RIIb, c, CD32, FCG2, IGFR2, CDw32

Source

• Human CD32b/c, His Tag (CDB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1 ). In the region Ala 46 - Pro 217, the AA sequence of Fc gamma RIIB and Fc gamma RIIC are homologus.
• Predicted N-terminus: Ala 46

Molecular Characterization

• This protein carries a polyhistidine tag at the C-terminus
• The protein has a calculated MW of 20.4 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin

• Less than 1.0 EU per μg by the LAL method.

Purity

• >95% as determined by SDS-PAGE.
• >90% as determined by SEC-MALS.

Formulation

• Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Contact us for customized product form or formulation.

Reconstitution

• Please see Certificate of Analysis for specific instructions.
• For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

• For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Please avoid repeated freeze-thaw cycles.
• This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 12 months under sterile conditions after reconstitution.

SDS-PAGE

Human CD32b/c, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR

Immobilized Human CD32b/c, His Tag (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 15.1 μM as determined in a SPR assay (Biacore T200) (QC tested).

Rituximab captured on Protein A Chip can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 3.56 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI

Loaded Human CD32b/c, His Tag (Cat. No. CDB-H5228) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 4.30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

SEC-MALS

The purity of Human CD32b/c, His Tag (Cat. No. CDB-H5228) is more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Immobilized Rituximab on CM5 Chip, can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 5.5 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Rituximab immobilized on CM5 Chip can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 4.09 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Loaded Rituximab on FAB2G Biosensor, can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 5.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).