CelLytic™ Y Plus Kit for enzymatic yeast cell lysis ยี่ห้อ Sigma

PROPERTIES

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

DESCRIPTION

Application

The kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris, and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immuno­reactivity and biological activity.

The cell extract is compatible with:

·       Reporter gene expression assays (e.g., β-galactosidase, alkaline-phosphatase)

·       Immunoassays (Western blots, immunoprecipitation)

·       Affinity based purification (e.g. FLAG, glutathione S- transferase (GST) and histidine-tagged fusion proteins)

·       DNA-protein interaction assay (e.g., gel-shift)

·       Coomassie and silver staining

The kit is suitable for spheroplast formation and protein extraction from 10-40 grams of yeast cell pellet.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC