GoTaq® MDx Hot Start Polymerase

Inhibits Polymerase Activity at Temperatures below 70°C

• Manufactured under cGMP
• High-concentration and glycerol-free formulations also available
• All formulations supplied at ≥5u/μl, except for high-concentration formulation, which is supplied at ≥50u/μl
• Custom formats and formulations

Consistent and Robust Amplification

GoTaq® MDx Hot Start Polymerase contains GoTaq® MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94–95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because primer-dimer and secondary products are eliminated or minimized. In some cases, hot-start PCR may improve yields. The glycerol-free product formulation is further purified to remove glycerol, making the enzyme suitable for further manufacture processing and lyophilization.

• Manufactured under cGMP using validated equipment, processes and method under a certified Quality System to ensure consistent product performance lot-to-lot.
• QC-tested for bacterial, fungal and mammalian DNA to ensure low DNA contamination.
• Flexible product formats and custom formulations available.

GoTaq® MDx Hot Start Polymerase is a general purpose reagent intended for general laboratory use. It is suitable for use as a component of molecular diagnostic assays, where applicable country laws allow. Separate license or royalty payments not required. This product alone does not provide any diagnostic result.

ItemPart #SizeConcentrationAvailable Separately

MgCl₂A351B3 × 750μl25mMView Product

GoTaq® MDx Hot Start PolymeraseD600B1 × 500u

5X Colorless GoTaq® Flexi BufferM890A4 × 1mlView Product

5X Green GoTaq® Flexi BufferM891A4 × 1mlView Product

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