3,3'-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride, for Membrane Applications ยี่ห้อ Sigma

PROPERTIES

Quality Level

200

form

liquid

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

DESCRIPTION

Application

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.

·       in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining[1]

·       in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi[2]

·       in immunoperoxidase staining of lung tissue[3]

The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.

Biochem/physiol Actions

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.[4]

Packaging

Set consists of 4 x 100 ml Solution A and 15 ml Solution B.

Caution

Stable for up to 18 mo. when stored at 2-8 °C in the original container.

Quantity

Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.