GloSensor™ Technology

Live-Cell Biosensors for cAMP, cGMP or Protease Activity

• Biosensors enable more complete analysis of receptor biology
• Simple, scalable assay protocol
• High Z´ and large signal:background ratio

Luciferase Biosensor for Real-Time Detection of Signaling Events

GloSensor™ technology provides a platform of flexible luciferase-based biosensors for real-time detection of signaling events in live cells with sensitivity, linearity and specificity.

GloSensor™ luciferase is circularly permuted, joining the wild-type N- and C-termini with a polypeptide or protein domain and creating engineered termini elsewhere within the structure. Analyte binding or protease cleavage leads to a conformational shift in the biosensor that promotes large increases in luminescence activity. The magnitude of the luminescence increase is directly proportional to the amount of analyte or protease activity present.

ItemPart #Size

pGloSensor™-20F cAMP Plasmid

E117A1 × 20μg

แหล่งที่มา : https://worldwide.promega.com/products/cell-signaling/gpcr-signaling/glosensor-camp-cgmp-protease-biosensors/?catNum=E1171