pCI-neo Mammalian Expression Vector

Constitutively Express Cloned Insert for Transient or Stable Transfection

• Select for stably transfected cells with neomycin phosphotransferase gene
• Increase the steady-state level of RNA with the late SV40 polyadenylation signal approximately fivefold more than the early SV40 polyadenylation signal
• Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate ssDNA in E. coli using the f1 origin of replication

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression.

This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Multiple cloning sites exist for easy insertion of cDNA.

References

1. Brinster, R.L. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 836–40.
2. Choi, T. et al. (1991) Mol. Cell. Biol. 11, 3070–4.
3. Palmiter, R.D. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 478–82.
4. Carswell, S. and Alwine, J.C. (1989) Mol. Cell. Biol. 9, 4248–58.

ItemPart #Size

pCI-neo Mammalian Expression Vector

E184A1 × 20μg

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